Released on: October 23, 2008, 5:30 am

Press Release Author: The Scott Partnership

Industry: Biotech

Press Release Summary: SANTA BARBARA, CALIFORNIA (October, 23, 2008) - Wyatt
Technology, the world leader in solutions for absolute macromolecular characterization, has published a new application note to demonstrate the unique
capability of its DynaPro Plate Reader dynamic light scattering (DLS) instrument for
achieving rapid detection and characterization of protein aggregates. The DynaPro
Plate Reader was able to perform DLS measurements on samples within the wells of a
well plate, without removing the samples or physically introducing any substances
into the wells. As a result, this innovative instrument was able to allow for
programmed measurements of many solution conditions quickly and easily with minimum
user intervention.

Press Release Body: DYNAPRO DLS PLATE READER FROM WYATT TECHNOLOGY PROVIDES RAPID
DETECTION AND CHARACTERIZATION OF PROTEIN AGGREGATES

SANTA BARBARA, CALIFORNIA (October, 23, 2008) - Wyatt Technology, the world leader
in solutions for absolute macromolecular characterization, has published a new
application note to demonstrate the unique capability of its DynaPro Plate Reader
dynamic light scattering (DLS) instrument for achieving rapid detection and
characterization of protein aggregates. The DynaPro Plate Reader was able to perform
DLS measurements on samples within the wells of a well plate, without removing the
samples or physically introducing any substances into the wells. As a result, this
innovative instrument was able to allow for programmed measurements of many solution
conditions quickly and easily with minimum user intervention.

Protein systems often exhibit aggregation as a result of changes in environmental
conditions, including changes in formulation pH, ionic strength, concentration,
series of freeze-thaw cycles and forced aging.
Research has demonstrated that aggregation of proteins is closely related and
perhaps causes several neurodegenerative disorders in humans such as Alzheimer's,
Parkinson's, Huntington's disease, amyotrophic lateral sclerosis and prion diseases.
As a consequence, much effort has been focused on determining the specific
conditions that lead to aggregation. DLS has proven a powerful and reliable
technique for this type of analysis, being capable of performing fast and accurate
measurements of multiple samples, thus clearly establishing aggregation conditions.

An experiment was developed to demonstrate the unique analytical capabilities of
DLS. For this particular application, protein samples with varying degrees of
aggregation were generated by heat stressing Bovine Serum Albumin (BSA) at 60C.
Aliquots were removed from the solution every five minutes for a total time period
of 175 minutes. Proteins were expected to exhibit increased aggregation with
increased time held at 60C. Triplicate 30 ?L aliquots of each condition were placed
into a low volume 384 well plate, resulting in 108 aliquots for the 36 different
exposure times.
Following sample collection and plate loading, the plate was placed into a Wyatt
DynaPro DLS Plate Reader with the sample space held at 5C. The plate was then
stored for 13 days at 5C and re-measured at 5C. Including movement between wells
and auto-adjustment of laser power and detector attenuation settings, the total
measurement time for the plate was only 50 minutes.

The results of the experiment clearly demonstrate that the average size of the
samples in solution increase with exposure time, resulting in longer correlation
times. The increased hydrodynamic radius and intensity of scattered light were
clearly indicative of increased aggregation of proteins with increased exposure to
60C. Aggregation was not found to evolve significantly over two weeks' storage at
5C.

For more information on Wyatt Technology's DynaPro DLS Plate Reader or to obtain a
copy of this application note, please visit www.wyatt.com or e-mail info@wyatt.com

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About Wyatt
Based in Santa Barbara, California, Wyatt Technology is the world's leading provider
of instruments for absolute macromolecular characterization. With over 40 years'
experience developing multi-angle light scattering detectors, working with customers
in the biotechnology, chemical, petrochemical, pharmaceutical, academic and
government arenas, Wyatt prides itself on its entrepreneurial spirit, and the
uniqueness of its offerings. The Company's groundbreaking technology and
uncompromising levels of customer care make Wyatt the global hallmark in its field.
For more information, please visit www.wyatt.com

For further press information please contact: Laura Browne, The Scott Partnership, 1
Whiteside, Station Road, Holmes Chapel, Cheshire. CW4 8AA United Kingdom Tel: + 44
1477 539539 Fax: +44 1477 539540 mail to: wyatt@scottpr.com

Web Site: http://

Contact Details: For further press information please contact: Laura Browne, The
Scott Partnership, 1 Whiteside, Station Road, Holmes Chapel, Cheshire. CW4 8AA
United Kingdom Tel: + 44 1477 539539 Fax: +44 1477 539540 mail to:
wyatt@scottpr.com